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SRX10000106: Canine_MUO_CSF_4
1 ILLUMINA (Illumina MiSeq) run: 729,100 spots, 306.8M bases, 193.9Mb downloads

Design: RNA was extracted with TRIzol (Thermo Fischer Scientific) and reverse transcribed to cDNA with non-ribosomal hexamers using Superscript IV (Thermo Fischer Scientific) using non-ribosomal hexamers. Second-strand cDNA synthesis and random amplification of samples was performed as previously described. A DNA library was constructed using the NexteraXT protocol (Illumina) with NGS performed on an Illumina MiSeq system using MiSeq Reagent kit V3 (300 x 2 cycles; Illumina).
Submitted by: University of Veterinary Medicine Hannover
Study: Canine Meningoencephalitis of unknown origin, the search for infectious agents via deep sequencing
PRJNA698629 • SRP304169 • All experiments • All runs
show Abstracthide Abstract
Meningoencephalitis of unknown origin (MUO) describes a group of meningoencephalitides in dogs and cats with a hitherto unknown trigger. It is postulated that a known or unknown pathogen might be associated with the initiation and/or progression of this disease. In this study, we have performed next generation sequencing and analysis on fresh-frozen cerebrospinal fluid samples of six dogs with MUO in the acute phase of the disease.
Sample: Canine_MUO_CSF_4
SAMN17730367 • SRS8169882 • All experiments • All runs
Organism: canine metagenome
Library:
Name: NGS_16004
Instrument: Illumina MiSeq
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM PCR
Layout: PAIRED
Runs: 1 run, 729,100 spots, 306.8M bases, 193.9Mb
Run# of Spots# of BasesSizePublished
SRR13605965729,100306.8M193.9Mb2021-02-02

ID:
13102588

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